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For fast, precise cutting of nitrocellulose and other membranes after immunoblots, polysaccharide, lipid and nucleic acid transfer processes onto an immobilising matrix.
 

The Accutran Strip Cutter is a precision instrument that cuts nitrocellulose and other membranes into strips. After the transfer of proteins, polysaccharides, lipids or nucleic acids to an immobilising matrix the complete and uniform cutting process maximises membrane utilisation and minimises waste.
 

Western blot immunoassays (based on the methods of Tang and co-workers, 1983 and 1985) include the electrophoretic transfer of separated antigens to a membrane (eg S&S NC nitrocellulose, BA83, 0.2 µm). This is then cut into strips and can now be used for various tests, such as the quantification of serum antibodies. The Accutran Strip Cutter efficiently and precisely increases the utilisation of the membrane and significantly reduces the membrane cutting time.
 

Accutran Cutters are available in different cutting strip width ranging from 2.5, 3, 4 or 5 mm. Each of these modules can cut membrane sheets up to 20 x 25 cm. The dry membrane sheet is placed on the cutting surface of the device and the cutting cassette is pulled over it. The result is a series of uniform strips with an uncut upper corner of 1.27 cm, which is supposed to make handling easier. Individual strips can then be removed directly from the leaf and used, for example, for immune incubations.
 

All four corner points of the razor blades within the blade cassettes can be used. This means that if the blades become dull due to frequent use, they can still be used in three other positions, which ultimately corresponds to four times the use. The blade cassettes can be obtained from Gasser Apparatebau. It is advisable to have a stock of razor blades in order to ensure an undisturbed work process.

Characteristics

• Precise, uniform cutting of membrane strips

• Increased efficiency when using membranes

• Inexpensive use of antigens, antibodies and other reagents

• Time savings compared to the hand-cut variant in the laboratory area

• Steel blades and anodised aluminum cutting plates that are resistant to most reagents